Urinary excretion of organosulfur compounds after acute ingestion of black onion.

Onion (Allium cepa L.) and its newly derived product "black onion" are characterised by the presence of compounds with potential bioactivity, particularly organosulfur compounds (OSCs). However, little is known about the metabolism, distribution, and excretion of these compounds as they pass through the gastrointestinal tract. This study monitored healthy subjects after an acute intake of black onion and analysed the excretion of OSCs using UHPLC-HRMS. A total of 31 OSCs were detected in urine after the acute ingestion of black onion, the main components being S-methyl-L-cysteine sulfoxide (methiin) (13.6 ± 3.9 µmol), isoalliin (12.4 ± 4.7 µmol) and S-propyl-L-cysteine (deoxypropiin) (3.1 ± 0.7 µmol). Moreover, N-acetylated metabolites of the major OSCs detected in black onion, namely, N-acetyl-S-(1-propenyl)-L-cysteine sulfoxide (NAS1PCS) and N-acetyl-S-(1-propenyl)-L-cysteine (NAS1PC), were found in urine after black onion consumption. The N-acetylation reaction takes place in the kidneys and liver, and metabolism pathways are proposed to explain the excretion of OSCs in urine. The basis of the identification of OSCs as urinary metabolites after black onion consumption is described for the first time and provides the basis for further research.

Bioavailability of Organosulfur Compounds after the Ingestion of Black Garlic by Healthy Humans.

The consumption of black garlic has been related to a decreased risk of many human diseases due to the presence of phytochemicals such as organosulfur compounds (OSCs). However, information on the metabolization of these compounds in humans is limited. By means of ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry (UHPLC-HRMS), this study aims to determine the OSCs and their metabolites excreted in urine 24 h after an acute intake of 20 g of black garlic by healthy humans. Thirty-three OSCs were identified and quantified, methiin (17,954 ± 6040 nmol), isoalliin (15,001 ± 9241 nmol), S-(2-carboxypropyl)-L-cysteine (8804 ± 7220 nmol) and S-propyl-L-cysteine (deoxypropiin) (7035 ± 1392 nmol) being the main ones. Also detected were the metabolites N-acetyl-S-allyl-L-cysteine (NASAC), N-acetyl-S-allyl-L-cysteine sulfoxide (NASACS) and N-acetyl-S-(2-carboxypropyl)-L-cysteine (NACPC), derived from S-allyl-L-cysteine (SAC), alliin and S-(2-carboxypropyl)-L-cysteine, respectively. These compounds are potentially N-acetylated in the liver and kidney. The total excretion of OSCs 24 h after the ingestion of black garlic was 64,312 ± 26,584 nmol. A tentative metabolic pathway has been proposed for OSCs in humans.

Effects of colonic fermentation on the stability of fresh and black onion bioactives.

The health properties related to onion intake are attributed mainly to the presence of bioactive compounds, particularly phenolic and organosulfur compounds (OSCs). The aim of this study was to investigate, for the first time, the effect of an in vitro colonic fermentation on the stability of phenolic and OSCs of fresh and black onion by ultra-high-performance liquid chromatography coupled with mass spectrometry with a linear ion trap (UHPLC-LIT-MS). Throughout colonic fermentation, fresh onion showed an increase in the total phenolic content of 45%, mainly due to an increase in the content of the flavonoid family, while the OSCs remained stable along the fermentation. Black onion presented a different behaviour, showing significant decreases in total (poly)phenol and OSC content, 22 and 48%, respectively. The main compounds found after the in vitro colonic fermentation of fresh onion were isorhamnetin (141 µmol L-1), quercetin (95 µmol L-1), 3,4-dihydroxybenzoic acid (53 µmol L-1), methionine sulfoxide (100 µmol L-1) and S-allylcysteine (SAC) (21.7 µmol L-1), whereas 3,4-dihydroxybenzoic acid (70 µmol L-1), 4-hydroxyphenylacetic acid (68 µmol L-1), methionine sulfoxide (82 µmol L-1) and S-propylmercapto-L-cysteine (SPMC) (10.1 µmol L-1) accounted for the highest concentrations of phenolics and OSCs in fermented black onion. These compounds, presumably present for their absorption and action at the colonic level, could be related to the health benefits of regular consumption of fresh and black onion.

In Vitro Colonic Fermentation of (Poly)phenols and Organosulfur Compounds of Fresh and Black Garlic.

The beneficial properties associated with garlic consumption have been related to the presence of bioactive compounds including (poly)phenols and organosulfur compounds (OSCs). This study aims to assess the effect of in vitro colonic fermentation on fresh and black garlic by determining the transformation of these compounds through ultrahigh-performance liquid chromatography coupled to mass spectrometry with a linear ion trap (uHPLC-LIT-MS). Colonic fermentation had a similar influence on the phenolic content of fresh and black garlic, with total respective decreases of 43.8% and 41.7%. Meanwhile, fermentation resulted in a significant decrease (33%) in OSCs in black garlic. Compounds such as 4-hydroxybenzoic acid, S-allylcysteine (SAC), and methionine sulfoxide were the phenolic compounds and OSCs with the highest concentration in fresh and black garlic after the in vitro fermentation. These compounds, potentially present at the colonic level, might be responsible for the systemic health benefits associated with the consumption of black and fresh garlic.

Antioxidant Activity and Bio-Accessibility of Polyphenols in Black Carrot (Daucus carota L. ssp. sativus var. atrorubens Alef.) and Two Derived Products during Simulated Gastrointestinal Digestion and Colonic Fermentation.

Black carrot has been attracting increasing thanks to its high bioactive compound content. This study presents the polyphenol bio-accessibility of black carrot and two derived products (black carrot snack (BC snack) and black carrot seasoning (BC seasoning)) after in vitro gastrointestinal digestion and colonic fermentation. Additionally, antioxidant activity was measured by 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS), 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and oxygen radical absorbance capacity (ORAC) assays. Nine flavonoids and eight anthocyanins were determined by ultra high-performance liquid chromatography high resolution mass spectrometry (UHPLC-HRMS) analysis, the predominant compounds being the hydroxycinnamic acids 3-O-feruloylquinic acid, 4-O-feruloylquinic acid and chlorogenic acid. The BC snack (108 µmol/g DW) presented the highest total polyphenol content, followed by BC seasoning (53 µmol/g DW) and black carrot (11.4 µmol/g DW). The main polyphenols still bio-accessible after in vitro digestion were the hydroxycinnamic acids, with mean recovery rates of 113 % for black carrot, 69% for BC snack and 81% for BC seasoning. The incubation of black carrot and its derived products with human faecal bacterial resulted in the complete degradation of anthocyanins and in the formation of mainly 3-(4'-hydroxyphenyl)propanoic acid as the major catabolic event. In conclusion, our results suggest that the black carrot matrix impacts significantly affects the bio-accessibility of polyphenols and, therefore, their potential health benefits.

Covid 19: Eating behavior changes related to individual and household factors during the COVID-19 lockdown in Spain

To analyze the influence of individual and household factors on eating behavior (EB) and other determinants related to eating during the home lockdown in the Covid-19 pandemic. Method: Online survey (April 17 to May 10, 2020) to collect sociodemographic information, health, and various EB attitudes. Statistical analysis of the factors: country, setting, sex, BMI classification, lockdown period, a household with children under 15 years, nutritional needs, age groups, type and size household, monthly income, and religion. Results: 1055 households participated. 75% have modified their eating habits, with differences due to being overweight or obese in the person surveyed (p <0.05). Changes in EB and other lifestyles are influenced by household structure (p <0.001) and the effects that the pandemic has had on the economy of families (p <0.001). Compared to those who do not have them, households with children tend to plan much more meals, spend more time eating, seek a healthier diet, increase the number of daily meals, and look more at labeling. In contrast, people who live alone have worsened the hourly routines of the main meals. Conclusions: Confinement has modified eating behavior differently depending on the individual and domestic factors analyzed(AU)

Analizar la influencia de factores individuales y del hogar sobre el comportamiento alimentario (CA), y otros determinantes relacionados con la alimentación, durante el confinamiento domiciliario en la pandemia por Covid-19. Método: Encuesta online (17 abril al 10 de mayo de 2020) para recopilar información sociodemográfica, de salud y diversas actitudes del CA. Análisis estadístico sobre los factores: país, ámbito, sexo, IMC, tiempo de confinamiento, hogar con menores de 15 años, necesidades nutricionales, grupos de edad, tipo y tamaño del hogar, ingresos y religión. Resultados: Participaron 1055 hogares. El 75% ha modificado sus hábitos alimentarios, con diferencias por sobrepeso u obesidad de la persona encuestada (p<0.05). Los cambios en el CA y otros estilos de vida están influenciados por la estructura del hogar (p<0.001) y los efectos que la pandemia ha tenido sobre la economía de las familias (p<0.001). Los hogares que tienen hijos/as, respecto a los que no los tienen, tienden a planificar mucho más las comidas, dedicar más tiempo a la alimentación, procuran una alimentación más saludable, incrementan el número de comidas diarias y miran más el etiquetado. Por el contrario, las personas que viven solas han empeorado las rutinas horarias de las principales comidas. Conclusiones: El confinamiento ha modificado de manera diferenciada el comportamiento alimentario en función de los factores individuales y domésticos analizados(AU)

Changes in the Organosulfur and Polyphenol Compound Profiles of Black and Fresh Onion during Simulated Gastrointestinal Digestion.

This study aims to determine the changes in, and bioaccessibility of, polyphenols and organosulfur compounds (OSCs) during the simulated gastrointestinal digestion of black onion, a novel product derived from fresh onion by a combination of heat and humidity treatment, and to compare it with its fresh counterpart. Fresh and black onions were subjected to in-vitro gastrointestinal digestion, and their polyphenol and OSC profiles were determined by ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry (UHPLC-HRMS). Although to a lesser extent than in the fresh onion, the phenolic compounds in the black variety remained stable during the digestion process, presenting a higher bioaccessibility index (BI) with recovery corresponding to 41.1%, compared with that of fresh onion (23.5%). As for OSCs, apart from being more stable after the digestion process, with a BI of 83%, significantly higher quantities (21 times higher) were found in black onion than in fresh onion, suggesting that the black onion production process has a positive effect on the OSC content. Gallic acid, quercetin, isorhamnetin, and ɣ-glutamyl-S-(1-propenyl)-L-cysteine sulfoxide were the most bioaccessible compounds in fresh onion, while isorhamnetin, quercetin-diglucoside, ɣ-glutamyl-S-methyl-L-cysteine sulfoxide and methionine sulfoxide were found in black onion. These results indicate that OSCs and polyphenols are more bioaccessible in black onion than in fresh onion, indicating a positive effect of the processing treatment.

Risk Assessment of Cd, Cu, and Pb from the consumption of hunted meat: red-legged partridge and wild rabbit.

The objective was to assess that potential health risk from Cd, Cu, and Pb, through the consumption of hunted red-legged partridge and wild rabbit meat, with special focus on the population of hunters and their relatives. Mineral content was analyzed by atomic absorption methods (F-AAS for Cu and GF-AAS for Cd and Pb) after microwave digestion of lyophilized samples. The average concentrations of these elements were 0.008 and 0.01 mg/kg for Cd; 1.41 and 1.63 mg/kg for Cu and 0.98 and 1.28 mg/kg for Pb in wild rabbit and red-legged partridge meat respectively. The dietary, risk assessment was performed by assuming two intake scenarios based on the obtained results of the survey on game meat consumption and the current maximum recommended intakes of Cd, Cu, and Pb, and then, the hazard quotients (THQ and TTHQ) were calculated. The data show that exposure to these metals from eating red-legged partridge and wild rabbit meat from a hunting provenance is relatively low and generally greater in the hunter population. The risk assessment revealed that moderate or low consumption of meat of these species does not offer a significant public health risk. Moreover, hazard quotients values for these metals of red-legged partridge and rabbit meat consumption in hunters and nonhunters are below 1. However, a high Pb content in the meat of these species and a high consumption may pose a greater health risk to hunters.

In Vitro Gastrointestinal Digestion and Colonic Catabolism of Mango (Mangifera indica L.) Pulp Polyphenols.

Mango (Mangifera indica L.), a fruit with sensorial attractiveness and extraordinary nutritional and phytochemical composition, is one of the most consumed tropical varieties in the world. A growing body of evidence suggests that their bioactive composition differentiates them from other fruits, with mango pulp being an especially rich and diverse source of polyphenols. In this study, mango pulp polyphenols were submitted to in vitro gastrointestinal digestion and colonic fermentation, and aliquots were analyzed by HPLC-HRMS. The main phenolic compounds identified in the mango pulp were hydroxybenzoic acid-hexoside, two mono-galloyl-glucoside isomers and vanillic acid. The release of total polyphenols increased after the in vitro digestion, with an overall bioaccessibility of 206.3%. Specifically, the most bioaccessible mango polyphenols were gallic acid, 3-O-methylgallic acid, two hydroxybenzoic acid hexosides, methyl gallate, 3,4-dihydroxybenzoic acid and benzoic acid, which potentially cross the small intestine reaching the colon for fermentation by the resident microbiota. After 48 h of fecal fermentation, the main resultant mango catabolites were pyrogallol, gallic and 3,4-dihydroxybenzoic acids. This highlighted the extensive transformation of mango pulp polyphenols through the gastrointestinal tract and by the resident gut microbiota, with the resultant formation of mainly simple phenolics, which can be considered as biomarkers of the colonic metabolism of mango.

Waist Circumference as a Preventive Tool of Atherogenic Dyslipidemia and Obesity-Associated Cardiovascular Risk in Young Adults Males: A Cross-Sectional Pilot Study.

Although the correlation coefficient between body mass index (BMI) and poor lipid profile has been reported, representing a cardiovascular risk, the need to find new early detection markers is real. Waist circumference and markers of atherogenic dyslipidemia are not usually measured in medical review appointments. The present study aimed to investigate the relationship between central adiposity and cardiovascular risk. This was a cross-sectional pilot study of 57 young males (age: 35.9 ± 10.85, BMI: 32.4 ± 6.08) recruited from community settings and allocated to non-obese or obese attending to their waist circumference. Total cholesterol (TC), high-density lipoproteins (HDL-C), and low-density lipoproteins (LDL-C) cholesterol and triglycerides (TG) were measured from plasma samples. Patients with at least 100 cm of waist circumference had significantly increased TC, LDL-C, non-HDL-C, and triglycerides and lower levels of HDL-C. The three atherogenic ratios TC/HDL-C, LDL-C/HDL-C, and TG/HDL-C were all optimal in non-obese patients. LDL-C/HDL-C and TG/HDL-C were significantly higher and over the limit when assessing for atherogenic dyslipidemia. The number of patients at risk for cardiovascular events increases 2.5 folds in obese compared to non-obese. Measurement of waist circumference could be adopted as a simpler valid alternative to BMI for health promotion, to alert those at risk of atherogenic dyslipidemia.

Bioaccessibility of Bioactive Compounds of 'Fresh Garlic' and 'Black Garlic' through In Vitro Gastrointestinal Digestion.

Numerous studies have reported health benefits associated with the consumption of fresh and black garlic, which are characterized by the presence of polyphenols and organosulfur compounds (OS). This study aims to analyze the bioaccessibility of the bioactive compounds in fresh and black garlic after in vitro gastrointestinal digestion by monitoring the individual profile of these compounds by ultra-high-performance liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-HRMS). Polyphenols decreased from the beginning of the digestive process, is mainly affected during intestinal digestion. Regarding the OS, the S-alk(en)yl-L-cysteine (SACs) derivatives were more influenced by the acidic conditions of the gastric digestion, while the γ-glutamyl-S-alk(en)yl-L-cysteine (GSAk) derivatives were more susceptible to intestinal digestion conditions in both the fresh and black garlic samples. In conclusion, after in vitro gastrointestinal digestion, the compounds with the highest bioaccessibility were vanillic acid (69%), caffeic acid (52%), γ-glutamyl-S-methyl-L-cysteine sulfoxide (GSMCS) (77%), and S-allylmercapto-L-cysteine (SAMC) (329%) in fresh garlic. Meanwhile, in black garlic, the main bioaccessible compounds were caffeic acid (65%), GSMCS (89%), methionine sulfoxide (262%), trans-S-(1-propenyl)-L-cysteine (151%), and SAMC (106%). The treatment (heating + humidity) to obtain black garlic exerted a positive effect on the bioaccessibility of OS compounds, 55.3% of them remaining available in black garlic, but only 15% in fresh garlic. Polyphenols showed different behavior regarding bioaccessibility.

Changes in the antioxidant activity and metabolite profile of three onion varieties during the elaboration of 'black onion'.

This study aims to investigate whether the heat treatment applied during the production of black onion, a novel derived product made from fresh onion, produces changes in the content of flavonoids, organosulfur compounds, organic acids, water soluble sugars and amino acids in three onion varieties ('Shallot', 'Chata' and 'Echalion'). The total flavonoid content decreased up to 12-fold in black onions compared with fresh onions while the quantities of isoalliin, the main organosulfur compound in black onions, drastically increased during the process. Moreover, the levels of fructose and glucose significantly increased during the elaboration process, contributing to the sweetness of black onions. The influence of heating on their antioxidant capacity showed a decreasing trend of the ORAC antioxidant activity of onion, while ABTS and DPPH did not show a clear tendency. These results present a comprehensive phytochemical characterization of black onions, highlighting the significant influence of the heating process on their phytochemical composition.

Trace Element Concentrations in Migratory Game Bird Meat: Contribution to Reference Intakes Through a Probabilistic Assessment.

The aim of this study was to determine Co, Cr, Cu, Fe, and Zn content in migratory game bird meat and its contribution to DRI through a probabilistic assessment. A total of 89 wild birds of three species, woodpigeon (Columba palumbus) (n = 25), common turtledove (Streptopelia turtur) (n = 19), and thrush (Turdus philomelos) (n = 45), were analyzed. Concentrations ranged between the following values: Fe: 35.6-39.6 mg/kg ww; Cu: 2.3-4.0 mg/kg ww; Zn: 11.0-18.6 mg/kg ww; and Cr: 43-51 µg/kg ww. Co content in this kind of meat was negligible. Significant differences were found for Cu and Zn content among species and for Cu, Fe, and Zn between breast and thigh muscle. Breast was the muscle with the highest Cu and Fe content, and thigh recorded the highest Zn levels for all species. Finally, a probabilistic assessment showed that consumption of a serving size between 100 and 200 g of game bird meat (80% breast + 20% thigh) that could meet the DRIs of Cu and Fe for 95th percentile. Then, game meat of these species could be considered as part of a healthy diet and a food with a high nutritional value.

Efecto de factores contextuales en la composición corporal de jugadores profesionales de fútbol. Un estudio retrospectivo / Effect of contextual factors on body composition in professional soccer players. A retrospective study

La exigencia de las demandas físicas en el fútbol ha evolucionado en los últimos años, poniendo de manifiesto la necesidad de investigar sobre aquellos aspectos que condicionan el rendimiento deportivo. Es por esto que el objetivo de este estudio fue describir la incidencia del entrenamiento individualizado, la compañía en las comidas, la raza y la demarcación sobre las variables antropométricas en jugadores de fútbol profesional. Para ello se desarrolló un estudio retrospectivo sobre 51 jugadores profesionales de la Segunda División B española durante las temporadas de 2015/2016, 2016/2017 y 2017/2018. La valoración antropométrica se realizó bajo las normas técnicas de medición recomendadas por el International Working Group of Kinanthropometry, adoptadas por la International Society for the Advancement of Kinanthropometry (ISAK). Los resultados revelaron que el entrenamiento individualizado y la compañía en las comidas fueron los factores que más influyeron sobre las variables antropométricas. Los valores de masa grasa y de masa muscular, y el sumatorio de pliegues son sensibles al efecto de la intervención sobre dichos factores. Los mayores niveles de interacción se producen entre la compañía en las comidas y el entrenamiento individualizado, y entre la demarcación y la compañía en las comidas. Considerando la composición corporal como un aspecto a tener en cuenta en el desarrollo del rendimiento, se concluye que la aplicación de ciertos contenidos del entrenamiento según las características individuales y el estilo de vida de los jugadores es un factor que posee una influencia significativa sobre los futbolistas profesionales

The requirements of physical demands in soccer have evolved in recent years, determining the need to investigate those aspects that condition athletic performance. The objective of this study was to describe the incidence of individualized training, company at meals, race, and demarcation on the anthropometric variables of professional soccer players since these four factors affect body composition, which is considered a predictor of performance and an indicator of lifestyle in these individuals. For this purpose, a retrospective study was developed in 51 professional players of the Spanish Football League Second Division B during the 2015/2016, 2016/2017, and 2017/2018 seasons. The anthropometric assessment was carried out under the technical standards of measurement recommended by the International Working Group of Kinanthropometry, adopted by the International Society for the Advancement of Kinanthropometry (ISAK). The results revealed that individualized training and company during meals were the factors that most influence exerted on the anthropometric variables that were collected. The values of fat mass and muscle mass, and the sum of fold measurements are sensitive to the effect of the intervention with these factors. The highest levels of interaction occurred between company during the meals and individualized training, and between demarcation and company during the meals. Considering body composition as an aspect to be taken into account in the development of performance, it should be considered that the application of certain training contents according to the individual characteristics and lifestyle of players are factors that may have a significant influence on professional soccer players

[Effect of contextual factors in body composition of professional soccer players. A retrospective study]. / Efecto de factores contextuales en la composición corporal de jugadores profesionales de fútbol. Un estudio retrospectivo.

INTRODUCTION: The requirements of physical demands in soccer have evolved in recent years, determining the need to investigate those aspects that condition athletic performance. The objective of this study was to describe the incidence of individualized training, company at meals, race, and demarcation on the anthropometric variables of professional soccer players since these four factors affect body composition, which is considered a predictor of performance and an indicator of lifestyle in these individuals. For this purpose, a retrospective study was developed in 51 professional players of the Spanish Football League Second Division B during the 2015/2016, 2016/2017, and 2017/2018 seasons. The anthropometric assessment was carried out under the technical standards of measurement recommended by the International Working Group of Kinanthropometry, adopted by the International Society for the Advancement of Kinanthropometry (ISAK). The results revealed that individualized training and company during meals were the factors that most influence exerted on the anthropometric variables that were collected. The values of fat mass and muscle mass, and the sum of fold measurements are sensitive to the effect of the intervention with these factors. The highest levels of interaction occurred between company during the meals and individualized training, and between demarcation and company during the meals. Considering body composition as an aspect to be taken into account in the development of performance, it should be considered that the application of certain training contents according to the individual characteristics and lifestyle of players are factors that may have a significant influence on professional soccer players.

INTRODUCCIÓN: La exigencia de las demandas físicas en el fútbol ha evolucionado en los últimos años, poniendo de manifiesto la necesidad de investigar sobre aquellos aspectos que condicionan el rendimiento deportivo. Es por esto que el objetivo de este estudio fue describir la incidencia del entrenamiento individualizado, la compañía en las comidas, la raza y la demarcación sobre las variables antropométricas en jugadores de fútbol profesional. Para ello se desarrolló un estudio retrospectivo sobre 51 jugadores profesionales de la Segunda División B española durante las temporadas de 2015/2016, 2016/2017 y 2017/2018. La valoración antropométrica se realizó bajo las normas técnicas de medición recomendadas por el International Working Group of Kinanthropometry, adoptadas por la International Society for the Advancement of Kinanthropometry (ISAK). Los resultados revelaron que el entrenamiento individualizado y la compañía en las comidas fueron los factores que más influyeron sobre las variables antropométricas. Los valores de masa grasa y de masa muscular, y el sumatorio de pliegues son sensibles al efecto de la intervención sobre dichos factores. Los mayores niveles de interacción se producen entre la compañía en las comidas y el entrenamiento individualizado, y entre la demarcación y la compañía en las comidas. Considerando la composición corporal como un aspecto a tener en cuenta en el desarrollo del rendimiento, se concluye que la aplicación de ciertos contenidos del entrenamiento según las características individuales y el estilo de vida de los jugadores es un factor que posee una influencia significativa sobre los futbolistas profesionales.

Influence of Variety and Storage Time of Fresh Garlic on the Physicochemical and Antioxidant Properties of Black Garlic.

Black garlic is made from the fresh kind, submitting it to a controlled temperature (~65 °C) and humidity (>85 °C) for a prolonged period of time. The aim of this study was to assess the differences in the process and in the final product as a result of employing three garlic varieties (Spanish Roja, Chinese Spring and California White), and to check the influence of the storage time on fresh garlic in the quality of the final product by using garlic obtained in two different agricultural seasons, that of the current year (2014) and of the previous one (2013). The results revealed some differences in the parameters analysed during the manufacturing of the black garlic from the three varieties used, and even according to the harvest in question. However, when comparing initial and final values of the samples, a very similar evolution in their acidity, reducing sugars, °Brix, pH, polyphenol content, and antioxidant capacity was noted.

Physicochemical Characterization and Biological Activities of Black and White Garlic: In Vivo and In Vitro Assays.

White and three types of black garlic (13, 32, and 45 days of aging, named 0C1, 1C2, and 2C1, respectively) were selected to study possible differences in their nutraceutic potential. For this purpose, garlic were physicochemically characterized (Brix, pH, aW, L, polyphenol, and antioxidant capacity), and both in vivo and in vitro assays were carried out. Black garlic samples showed higher polyphenol content and antioxidant capacity than the white ones. The biological assays showed that none of the samples (neither raw nor black garlic) produced toxic effects in the Drosophila melanogaster animal genetic model, nor exerted protective effects against H2O2, with the exception of the 0C1 black garlic. Moreover, only white garlic was genotoxic at the highest concentration. On the other hand, 0C1 black garlic was the most antigenotoxic substance. The in vivo longevity assays showed significant extension of lifespan at some concentrations of white and 0C1and 1C2 black garlic. The in vitro experiments showed that all of the garlic samples induced a decrease in leukemia cell growth. However, no type of garlic was able to induce proapoptotic internucleosomal DNA fragmentation. Taking into account the physicochemical and biological data, black garlic could be considered a potential functional food and used in the preventive treatment of age-related diseases. In addition, our findings could be relevant for black-garlic-processing agrifood companies, as the economical and timing costs can significantly be shortened from 45 to 13 days of aging.

Caracterización del consumo de alimentos e ingesta de nutrientes de población residente en las Islas Galápagos / Characterization of food consumption and nutrient intake of population resident in the Galapagos Islands

Las Islas Galápagos es una de las provincias de Ecuador con mayores índices de sobrepeso y obesidad en población adulta. Debido a las restricciones de producción de alimentos, la presión turística y otros factores sociales; la disponibilidad, el acceso y consumo de alimentos saludables y recomendables puede estar afectando al estado de seguridad alimentaria y nutricional de su población. Por ello, el objetivo de este estudio es analizar el consumo de alimentos de la población y su adecuación a las necesidades nutricionales. Sobre una muestra de 120 personas residentes en las Islas Galápagos se aplicaron 3 R24h. Los resultados de la valoración nutricional fueron comparados con las IDR, realizando un análisis factorial multivariante para analizar diferencias por grupo de edad o desempeño profesional. Se realizó un análisis de componentes principales para caracterizar el perfil de ingesta de nutrientes. Para energía y principios inmediatos se sobrepasan las recomendaciones, principalmente entre las mujeres, a la vez que se evidencia una ingesta inadecuada de fibra, iodo, ácido fólico y vitamina E. Respecto a los grupos de alimentos, el consumo medio de frutas y verduras frescas es muy escaso y el aporte proteico proviene en mayor medida de productos cárnicos (46%), seguido de pescado (24%) y arroz (17%). Los resultados de este estudio evidencian la necesidad de asegurar el acceso a alimentos frescos y saludables, en especial frutas y verduras, y promover la adopción de pautas nutricionales que promuevan un consumo adecuado de ciertos alimentos(AU)

The Galapagos Islands are one of the provinces of Ecuador with the highest rates of overweight and obesity among the adult population. Due to its restrictions on local production, tourist pressure and other social factors; availability, access and consumption to food is a problem that is related to the state of food and nutritional security of its population. Therefore, the objective of this study is to analyze the pattern of food consumption of healthy and recommended foods may be affecting the food insecurity or nutritional status of its population. Therefore, the objective of this study is to analyze the food consumption of the population and its adaptation to nutritional needs. On a sample of 120 people resident in the Galapagos Islands, 3 R24h were applied. The results of the nutritional assessment were compared with the RDI. A multivariate factor analysis has been performed to analyze differences by age group or professional performance. Principal component analysis (PCA) was performed to characterize the nutrient intake. Recommendations are exceeded for energy and immediate principles, mainly among women. On the other hand, there is an inadequate intake of fiber, iodine, folic acid and vitamin E. Regarding food groups, the average consumption of fresh fruits and vegetables is very low and protein intake comes mostly from meat products (46%), followed by fish (24%) and rice (17%). The results of this study demonstrate the need to ensure access to fresh and healthy foods, especially fruits and vegetables, and to promote the adoption of nutritional guidelines that promote adequate food intake(AU)

Wild mushroom consumption by pickers in the south of Spain: a probabilistic approach.

A survey has been made of 300 habitual consumers of the eight species of wild mushrooms most often consumed in the south of Spain. The eight species selected constitute over 95% of the intake of this food in the samples studied. The mean consumption per capita of mushrooms in Spain is of 10.4 kg/year, 8.6 kg of which are consumed during the season, which lasts from between 1 and 3 months. Male pickers from the Huelva province were those who presented the largest intake, their age group being highly influenced by the species. The consumption of each mushroom studied and the total intake were adjusted/fitted to exponential distributions. These distributions could be an effective tool for toxicological or nutritional studies since they permit the evaluation of exposure that makes it possible to calculate the probabilistic risk analysis and the contribution to the reference dietary intake, respectively, for this population group.

Game meat consumption by hunters and their relatives: a probabilistic approach.

This study aimed to estimate the consumption of meat and products derived from hunting by the consumer population and, specifically, by hunters and their relatives. For this purpose, a survey was conducted on the frequency of consuming meat from the four most representative game species in Spain, two of big game, wild boar (Sus scrofa) and red deer (Cervus elaphus), and two of small game, rabbit (Oryctolagus cuniculus) and red partridge (Alectoris rufa), as well as of processed meat products (salami-type sausage) made from those big game species. The survey was carried out on 337 habitual consumers of these types of products (hunters and their relatives). The total mean game meat consumption, per capita in this population group, is 6.87 kg/person/year of meat and 8.57 kg/person/year if the processed meat products are also considered. Consumption of rabbit, red partridge, red deer and wild boar, individually, was 1.85, 0.82, 2.28 and 1.92 kg/person/year, respectively. It was observed that hunters generally registered a larger intake of game meat, this being statistically significant in the case of rabbit meat consumption. Using probabilistic methods, the meat consumption frequency distributions for each hunting species studied were estimated, as well as the products made from big game species and the total consumption both of meat by itself and that including the products made from it. The consumption frequency distributions were adjusted to exponential ones, verified by the test suitable for it according to Akaike Information Criterion (AIC), Bayesian Information Criterion (BIC), the Chi-squared and Kolmogorov-Smirnov statistics. In addition, the consumption percentiles of the different distributions were obtained. The latter could be a good tool when making nutrition or contaminant studies since they permit the assessment of exposure to the compound in question.